BaseSpace Hub NGS Data Analysis (2024)

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Integrate, analyze, and discover with BaseSpace Sequence Hub

BaseSpace Sequence Hub is the Illumina cloud-based genomics computing environment for next-generation sequencing (NGS) data management and analysis. Sequencing labs can store and share sequencing data, and researchers can simplify and accelerate NGS data analysis with push-button tools. Labs can also set-up and monitor their sequencing runs in real time on any Illumina instrument. BaseSpace Sequence Hub can be accessed via an intuitive web-based interface or Linux-based command line tool. For customers who need a local solution, we offer BaseSpace Onsite Sequence Hub.

BaseSpace Hub NGS Data Analysis (1)

Register now for a BaseSpace Sequence Hub Basic
account, which includes 1 TB Storage.

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BaseSpace Sequence Hub Subscription Tiers

BaseSpace Sequence Hub subscription tiers enable labs of all sizes to achieve maximum efficiency with tight instrument integration and simplified collaboration. We have three tiers of offerings.

Basic

Professional

Enterprise

StorageUp to 1 TB11 TB plus pay as you go1 TB plus pay as you go
Compute30 Day Free Trial2 of 250 complimentaryCredits3250 complimentaryiCredits3 plus pay as you go250 complimentaryiCredits3 plus pay as you go
Premier Data Security and Privacy
Run Setup
Unlimited Monitoring
Unlimited Sharing4
AppsFree apps only
(after trial period expires)
AllAll
Comprehensive Security Framework
Multi-user Access
Bioinformatics Professional Services Support5 (8 hours)
(24 hours)
Multiple Workgroups
Private Domain and Single Sign-on
Supports customers in a HIPAA regulated environment
Access Control & Audit
PriceFreeContact a Sales RepContact a Sales Rep

1. Customers wanting to purchase additional storage must upgrade to Professional or Enterprise account
2. Customers wanting additional iCredits must upgrade to a Professional or Enterprise account
3. iCredits will be applied to users account upon launch of Compute Billing
4. Unlimited sharing and transfer apply only to Basic accounts under the 1 Tb storage quota.
5. Professional services are not included with the academic plans.
For Research Use Only. Not for use in diagnostic procedures.

Simplifying Bioinformatics to Speed Up Discovery

BaseSpace Sequence Hub is the only bioinformatics platform that is directly connected to all Illumina sequencing instruments*.

Stream sequencing runs and store data immediately.

  • With unlimited storage on the cloud, you never have to worry about running out of space**.
  • Monitor sequencing runs in BaseSpace Sequence Hub with Sequencing Analysis Viewer (SAV) charts.
  • Perform routine data analysis immediately with the push of a button.
  • Choose from more than 70 pre-packaged analysis options available from Illumina and third-party developers
  • Open platform allows you to upload your own pipeline as a public or private app

With BaseSpace Sequence Hub there is no need to set-up and maintain IT infrastructure to store and analyze your genomics data.

We offer scientific and technical consulting through our Services offering to help guide you through bioinformatics workflows. Bioinformatics Professional Services offers group and one-on-one sessions on topics including introduction to bioinformatics, how to use various BaseSpace Sequence Hub features to maximize productivity, in-depth discussions on specific NGS workflows, and assistance creating custom pipelines into BaseSpace Sequence Hub.

*HiSeqX does not connect to BaseSpace Onsite Sequence Hub
** Customers wanting to purchase additional storage must upgrade to Professional or Enterprise account

Explore the Benefits of BaseSpace Sequence Hub

See how our informatics platform offers a rapid, scalable, and secure NGS solution with minimal infrastructure investment.

View Infographic

Key Features

In addition to NGS data analysis, sample/run management, and cloud bioinformatics or onsite options, key features of BaseSpace Sequence Hub include:

  • Sequencing instrument integration: BaseSpace Sequence Hub is directly integrated with Illumina sequencing instruments. This integration streamlines real-time run monitoring and QC, and facilitates data sharing and transfer.
  • Simplified NGS data management and storage: BaseSpace Sequence Hub solves NGS data storage and management challenges with practically inexhaustible storage space in the cloud*.
  • Easy data sharing: Because the BaseSpace Sequence Hub cloud is accessible everywhere, data sharing is instantaneous. Easily share individual sequencing runs or entire projects with collaborators anywhere in the world with a few clicks.
  • Multiple user Interfaces: BaseSpace Sequence Hub offers an intuitive, easy-to-use web-based interface for managing your data. For those more comfortable with a command line interface, BaseSpace Command Line Interface and BaseMount enable navigation, download/upload, launching analyses (including automatic launch of applications) and enhanced automation.
  • Sample and Run Prep: The Prep tab is an intuitive, graphical environment for one-stop library and run preparation.

Learn More about BaseSpace Sequence Hub Features

* Customers wanting to purchase additional storage must upgrade to Professional or Enterprise account.

NGS Data Analysis Apps

BaseSpace Sequence Hub offers a wide variety of powerful NGS data analysis apps, including:

  • Illumina Core Apps: Developed or optimized and fully supported by Illumina
  • BaseSpace Lab Apps: Developed using an accelerated process to make them available to BaseSpace Sequence Hub users faster than conventional Illumina Apps, and provided as-is
  • Third-party apps: Developed and supported by a thriving ecosystem of third-party app providers in BaseSpace Sequence Hub

In addition, BaseSpace Sequence Hub enables users to develop custom bioinformatics apps in BaseSpace Sequence Hub. App developers are able to keep apps private, share with collaborators, or submit for publication.

Together, these apps cover the common data analysis methods used with Illumina sequencing data. These methods include RNA-Seq, exome /enrichment, amplicon, whole-genome sequencing (WGS), amplicon, de novo assembly, 16S metagenomics, and more.

Learn More about BaseSpace Apps

BaseSpace Onsite Sequence Hub

BaseSpace Onsite Sequence Hub is a local version of the BaseSpace Sequence Hub cloud bioinformatics solution that enables you to safely and securely store, share, and analyze NGS data from MiniSeq, MiSeq, MiSeqDx (in RUO mode), NextSeq 500, NextSeq 550, HiSeq 2500, HiSeq 3000, and HiSeq 4000 systems.

Learn More about BaseSpace Onsite Sequence Hub

Additional Resources

BaseSpace Sequence Hub Data Sheet View PDF Learn more about BaseSpace Sequence Hub highlights, tools, and applications.

Introduction to BaseSpace Sequence Hub View Video Learn how you can store and analyze your genomic research with ease, and effortlessly manage all of your sequencing runs.

Monitoring Instrument Performance View PDF BaseSpace Sequence Hub enables users to submit sequencing instrument operational data, so Illumina can monitor instrument performance and resolve unforeseen issues.

BaseSpace Sequence Hub White Paper View PDFBaseSpace Sequence Hub has a set of comprehensive policies and controls for data security and privacy.

BaseSpace Onsite Sequence Hub BaseSpace Hub NGS Data Analysis (2)View Video BaseSpace Onsite Sequence Hub allows users to access their next-generation sequencing data anywhere.

BaseSpace Apps Quick Guide View PDF

BaseSpace Sequence Hub Support Find user guides, app release notes, and additional technical information. Access BaseSpace Sequence Hub Support

Immuno-oncology Informatics Apps Guide View PDF Informatics tools enable discovery and analysis with standard Illumina sequencing applications.

BaseSpace Sequence Hub is intended for research applications and not for any diagnostic or clinical use. It is intended to be used with Illumina research products only.

BaseSpace Clarity LIMS

Sequencing Data Library

ForenSeq Universal Analysis Software

BaseSpace Hub NGS Data Analysis (2024)

FAQs

How do I requeue analysis in BaseSpace? ›

Use BaseSpace Sequence Hub to edit a sample sheet and requeue:
  1. After logging in to your BaseSpace account, select the RUNS tab at the top of the page.
  2. Select the blue hyperlink of the run you wish to requeue.
  3. Select the hourglass symbol drop-down menu, then select Requeue and then Sample Sheet.

How do you analyze NGS data? ›

The key steps for NGS data analysis are cleaning, data exploration, visualization, and deepening. Whether you perform genomic, gene expression, or methylation analyses, you can apply these steps in this article and some valuable tools to help you process high-throughput data.

What is a BaseSpace sequence hub? ›

BaseSpace Sequence Hub (BSSH) is a genomics cloud computing software that provides researchers with streamlined run management and intuitive analysis apps in a user-friendly format.

What is library prep in NGS? ›

Overview. Library preparation is the first step of next generation sequencing. It allows DNA or cDNA to adhere to the sequencing flow cell and allows the sample to be identified. Two common methods of library preparation are ligation-based library prep and tagmentation-based library prep.

How to requeue analysis? ›

Requeue analysis of a run using the MiSeq Reporter web interface
  1. From the MiSeq Reporter web interface, select the Analyses tab on the top left.
  2. Locate the run from the list of available runs in the Analyses tab, click on the checkbox next to the run name, then select Requeue.
May 6, 2024

How to requeue analysis local run manager? ›

From the Local Run Manager dashboard, select More Options (three vertical dots) next to the run you wish to requeue. Select Requeue. In the pop-up window, choose whether to Edit Setup or to Requeue with the original setup information.

How to download data from illumina BaseSpace? ›

After installing the BaseSpace downloader, select “All analysis data files including Variant data (VCF), Aligned data (BAM), and FASTQ” and click on the “Download your files” button. BaseSpace downloaded should launch automatically.

Is Illumina BaseSpace free? ›

BaseSpace Sequence Hub offers a limited 30-day free trial for new accounts. New free trial accounts have access to the following features. 1 TB free storage—Additional storage can be purchased with promotional iCredits.

What does fastq stand for? ›

FASTQ format is a text-based format for storing both a biological sequence (usually nucleotide sequence) and its corresponding quality scores. Both the sequence letter and quality score are each encoded with a single ASCII character for brevity. FASTQ format. Internet media type. text/plain.

What are the 4 steps of NGS? ›

NGS workflow basics

Next-generation sequencing involves four basic steps: extraction, library preparation, sequencing, and data analysis.

What is the workflow of NGS data analysis? ›

The next generation sequencing workflow consists of four key steps: nucleic acid extraction, library preparation, sequencing, and analysis. The choices you make at each step are critical for your experimental outcomes.

What is NGS for dummies? ›

Essentially you take your DNA sample and fragment it in to small pieces. The two strands are separated, and a controlled chemical reaction occurs to add the complementary DNA bases back, one at a time, to the single strand to create a double strand. A chemical or fluorescent signal is given off as each base is added.

How to requeue analysis MiSeq reporter? ›

From the MiSeq Reporter web interface, click Analyses. Locate the run from the list of available runs on the Analyses tab, and click the Requeue checkbox adjacent to the run name. If the run is not listed, confirm that the correct repository is specified using the Settings icon.

How to fix sample sheet in BaseSpace? ›

Fix Sample Sheet
  1. From a Run summary page, select the Status drop-down list, point to Requeue, and then select Sample Sheet. ...
  2. To make minor changes, edit the sample sheet in the sample sheet editor. ...
  3. To generate a new sample sheet or make complex changes, use the Illumina Experiment Manager.
May 8, 2024

How do you share data in Illumina BaseSpace? ›

To share a Run/Project using the Get Link option:
  • Log into BaseSpace.
  • Navigate to the Runs or Projects tab list on BaseSpace.
  • Select the check box next to Run Name or Project Name to be shared.
  • Select the "Share" button above the list .
  • Select the "Get Link" option, and Activate a link for the Run/Project.
May 6, 2024

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